Device

Part:BBa_M47007:Design

Designed by: Joshua Charles Woodard   Group: MIT BE 20.20 - S15   (2015-05-11)

Polystyrene Decomposer


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1477
    Illegal EcoRI site found at 3168
    Illegal EcoRI site found at 3418
    Illegal PstI site found at 1388
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1477
    Illegal EcoRI site found at 3168
    Illegal EcoRI site found at 3418
    Illegal PstI site found at 1388
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1477
    Illegal EcoRI site found at 3168
    Illegal EcoRI site found at 3418
    Illegal BglII site found at 3244
    Illegal XhoI site found at 31
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1477
    Illegal EcoRI site found at 3168
    Illegal EcoRI site found at 3418
    Illegal PstI site found at 1388
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1477
    Illegal EcoRI site found at 3168
    Illegal EcoRI site found at 3418
    Illegal PstI site found at 1388
    Illegal NgoMIV site found at 640
    Illegal NgoMIV site found at 3535
    Illegal AgeI site found at 235
    Illegal AgeI site found at 2164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2911


Design Notes

This device design is untested and barrows genes from a wide variety of bacteria to function. The hope is that any general chassis will be able to run these genes and produce these products effectively, but there are a lot of unknowns between the concept in question and the actual final product.


Source

The esterase gene comes from a publication by Henne, Schmitz et al. from the University of Goettingen. http://www.ncbi.nlm.nih.gov/nuccore/AF223648.1 The alcohol dehydrogenase gene comes from Arthrobacter; the gene sequence was decoded by J.E. Koenig of Dalhousie University http://www.ncbi.nlm.nih.gov/nuccore/FM995514.1 The alkane hydroxylase gene is derived from Alcanivorax borkumensis by A. Hara of Kamaishi Laboratories. http://www.ncbi.nlm.nih.gov/nuccore/AB110225.1 The BVMO gene is taken from Pseudomonas fluorescens by U.T. Bornscheuer of Greifswald University. http://www.ncbi.nlm.nih.gov/nuccore/226880370

References

Our inspiration for the enzymatic combination above comes from these sources: http://www.sciencedirect.com/science/article/pii/S0922338X97820132 http://link.springer.com/article/10.1007/s00253-006-0443-1/fulltext.html#Fig2